Device

Part:BBa_K561003:Design

Designed by: MA Keyue   Group: iGEM11_ZJU-China   (2011-09-29)


PfdhF+RFP+tetR+Pvgb+YFP+tetR+Ptet+CFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 680
    Illegal AgeI site found at 792
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We construct tetR to resist CFP expression under microaerobic and anaerobic conditions.


Source

vgb promoter comes from Vitreoscilla sp. and fdhF comes from E.coli.

References